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11.
Ikeda M Konnai S Onuma M Ishiguro N Goryo M Okada K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(4):425-432
Tumor necrosis factor-alpha (TNF-alpha) has been reported to be associated with the progression of lymphoproliferative neoplastic diseases and retroviral infections. Hence we examined immunohistochemically the expression patterns of TNF-receptors (TNF-RI and RII) on lymphoma cells derived from the 29 cases of enzootic bovine leukosis (EBL). Lymphomas obtained in 29 animals with EBL were histopathologically classified into three types: diffuse mixed type (10 cases), diffuse large type (9 cases), and diffuse large cleaved type (10 cases). Immunohistochemically using a monoclonal antibody to a bovine lymphocyte surface antigen, the lymphomas were classified into three phenotypes: B-1a (CD5+/CD11b+), B-1b (CD5-/CD11b+) and B-2 (conventional B) (CD5-/CD11b-). Interestingly, the lymphoma cells in all animals expressed TNF-RII, but not TNF-RI. Although, in EBL, lymphoma cells of which the histopathological and immunological property differs has been formed, the expression patterns of TNF-Rs had the universality in all lymphoma cells. TNF-RII, which induces cell proliferation, was expressed but TNF-RI, which induces cell apoptosis was not expressed on all lymphoma cells, suggesting that TNF-Rs play an important role in the malignant proliferation of B cells and formation of lymphomas in EBL. 相似文献
12.
The distinct properties of fluorous phases are practically useful for separation, purification, and reaction control in organic synthesis. Here, we report the formation of a liquid-like fluorous droplet, composed of 24 perfluoroalkyl chains confined in the interior of a 5-nanometer-sized, roughly spherical shell that spontaneously assembled in solution from 12 palladium ions and 24 bridging ligands. Crystallographic analysis confirmed the rigid shell framework and amorphous interior. Perfluoroalkanes can dissolve in this well-defined fluorous phase, whereas they can hardly dissolve in a surrounding polar organic solution, and their solubility (up to approximately eight perfluoroalkane molecules per spherical complex) can be finely controlled by tuning the length of perfluoroalkyl chains tethered to the shell. 相似文献
13.
Kaori KASUGA Maho HIGASHI Takahisa YAMADA Toshie SUGIYAMA Yukio TANIGUCHI Hiroaki IWAISAKI Kosuke HOMMA Yuuichi WAJIKI Yoshinori KANEKO Satoshi YAMAGISHI 《Animal Science Journal》2012,83(1):83-87
The Japanese crested ibis Nipponia nippon is a critically threatened bird. Accurate sexing is necessary to perform effective management of captive breeding toward a national project for a tentative release of the Japanese crested ibis on Sado Island. A PCR‐based sexing method targeting a 0.6 kb EcoRI fragment (EE0.6) sequence on W chromosome with AWS03 and USP3 primers has been developed for the Japanese crested ibis. However, the primers were selected from the EE0.6 sequences from bird species other than the Japanese crested ibis. In this study, we determined the W‐ and Z‐linked EE0.6 sequences in the Japanese crested ibis, and clarified Japanese crested ibis sequence mismatch in the binding sites of the primers. Further, we found no polymorphism in the primer binding sites among five founder birds for the Sado captive Japanese crested ibis population. These findings validated the PCR‐based sexing method with the AWS03 and USP3 as accurate molecular sexing methods of captive Japanese crested ibis on the Sado Island. Additionally, we designed a primer set for a novel PCR‐based sexing, based on the EE0.6 sequences obtained in this study. This novel sexing method may be useful for future ecological research following the release of Japanese crested ibis on Sado Island. This is the first report to show the EE0.6 sequences in Japanese crested ibis. 相似文献
14.
Noborio Kosuke Ito Yuki He Hailong Li Min Kojima Yuki Hara Hirofumi Mizoguchi Masaru 《Paddy and Water Environment》2018,16(1):81-87
Paddy and Water Environment - Hydraulic properties of soil play important roles in water and temperature regimes. Measuring hydraulic properties has been studied for decades in the laboratory and... 相似文献
15.
At 114 ranches in the Hidaka District of Hokkaido, a total of 1,118 Thoroughbred foals
that were born from January to June 2003 were examined at different times from May to
September of the same year to ascertain the occurrence of clubfoot. Clubfoot was seen in a
total of 179 foals (16%) at 89 ranches (78%). Also, 124 of the 179 foals (69.3%) had
clubfoot of grade II or higher, and it was found that grade I was likely to be overlooked
or rapidly advance. In the present study, the occurrence of clubfoot was higher than
expected in the investigated region. Hence, it is necessary to establish accurate
diagnostic techniques and clubfoot guidelines to minimize the impact. 相似文献
16.
Kosuke Izumitsu Syunichi Kimura Hajime Kobayashi Atsushi Morita Yoshimoto Saitoh Chihiro Tanaka 《Journal of General Plant Pathology》2010,76(4):254-260
Hydrophobins are small secreted proteins unique to filamentous fungi. In this study, we cloned and characterized the class
I hydrophobin gene BcHpb1 in the necrotrophic pathogen Botrytis cinerea. The BcHpb1 protein consisted of 117 amino acids. Similar to class I hydrophobins from other fungi, BcHpb1 contains eight
conserved cysteine residues. The hydropathy plot of the BcHpb1 amino acid sequence was characteristic of a class I hydrophobin.
These results indicated that the BcHpb1 gene encodes a class I hydrophobin. Vegetative growth of ΔBcHpb1 strains, null mutants of BcHpb1, was similar to that of the wild-type strain as were the conidiophores, conidia, appressoria and virulence on host plants.
However, adherence of ΔBcHpb1 strains to hydrophobic surfaces was greatly reduced, implying that BcHpb1 is important for the hydrophobicity of conidia
and that BcHpb1 may be required to adhere to plant surfaces under certain environmental conditions. 相似文献
17.
Satake S Nakamura C Minamide Y Kudo S Maeda H Chihaya Y Kamimura Y Miyajima H Sasaki J Goryo M Okada K 《Journal of toxicologic pathology》2010,23(2):75-83
To elucidate the effect of a large dose of di (2-ethylhexyl) phthalate (DEHP), a plasticizer and peroxisome proliferator-activated receptor-α (PPARα) agonist, on hepatic peroxisomes, we orally administered 1,000 mg/kg/day, once daily, to 3 male and 4 female cynomolgus monkeys for 28 days consecutively. Light-microscopic and electron microscopic examinations of the liver were carried out in conjunction with measurement of the hepatic fatty acid β-oxidation system (FAOS), carnitine acetyltransferase (CAT) and carnitine palmitoyltransferase (CPT) activities, which are peroxisomal and/or mitochondrial enzyme activities. Electron microscopically, enlargement of the mitochondria was observed with lamellar orientation of the cristae along the major axis. Although the number of peroxisomes showed a tendency to increase when compared with those in a biopsied specimen before treatment, no abnormality in morphology was observed. A slight increase in CPT activity was noted at termination. No changes were noted in hepatic FAOS or CAT activity. In conclusion, although repeated oral treatment of cynomolgus monkeys with a large dose of DEHP induced a subtle increase in the numbers of peroxisomes with slight enlargements of the mitochondria, this low-sensitivity response to peroxisome proliferators in cynomolgus monkeys was considered to be closer to the response in humans than that in rodents. 相似文献
18.
Ground-dwelling beetle community in beech forests of Sado Island,Japan: comparison with the mainland
Island ecosystems differ from mainland ecosystems in their long-term isolation. On Sado Island, Japan, cattle grazing in the
past gradually changed forests into grasslands, thereby altering the diversity of indigenous insects. We compared ground-dwelling
beetle diversity on Sado Island to that on the nearby mainland. Contrary to previous reports, the island isolation effect
resulted in lower γ and β diversity than on the mainland, because of the low species richness of beetles with low dispersal
abilities. Few flightless beetles could invade Sado Island, even when the beech forests on the island were relatively adjacent
to those on the mainland. Such a history of ecosystem formation could be the general pattern for continental-island mountain
forests that were never connected to those of the mainland. We did not observe low α diversity of ground-dwelling beetles,
suggesting that the cattle grazing history of the island has not changed environmental conditions for ground-dwelling beetles
enough to reduce their diversity. In addition, cattle grazing increases the abundance of merdivorous insects by increasing
the abundance of their food resource.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
19.
20.
Yusuke Kudo Kosuke Noborio Naoto Shimoozono Ryuki Kurihara Hayato Minami 《Paddy and Water Environment》2017,15(1):217-220
Many papers on measurements of greenhouse gases (GHGs) emission in rice paddies during a rice cropping season have been published. During a non-cropping season between Nov. and Apr., we investigated direct and indirect GHGs emissions in rice paddies. The indirect GHGs emission was evaluated as the amount of dissolved gases leaching from the paddy fields. Water management practices for the experiment were (1) continuous flooding (CF) and (2) non-flooding (NF). Although the direct CO2 emission in the CF treatment was remained nearly zero during the non-cropping period, direct CO2 emission in the NF treatment was continuously observed throughout the non-cropping period. The concentration of dissolved N2O in the NF treatment was below the detection limit of the instrument during the non-cropping period except immediately after the flooding and before the drainage. The concentration of dissolved N2O kept approximately 2 µg L?1 during the non-cropping period in the CF treatment. The direct CH4 emission and dissolved CH4 were not observed during the non-cropping period. Total gas emission in the NF treatment was 10 times as large as that in the CF treatment. Direct CO2 emission accounted for more than 90 % of the total emission in both treatments. 相似文献